Biotin-coupled mirna capture

Author: ylfg

August undefined, 2024

WebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... WebMay 31, 2024 · The biotin-coupled miRNA capture assay was performed as previously described. 17 In brief, miR-206 mimics and miRNA negative control were labeled with biotin at the 3′ end and transiently transfected into LoVo and HCT116 cells. After 24 hrs of culture, the cells were harvested and lysed in a lysis buffer, followed by incubation with ...

Capture and Identification of miRNA Targets by Biotin

WebJan 16, 2024 · RNA immunoprecipitation (RIP) and biotin-coupled miRNA capture. g for 20 min. Western blot analysis. The total protein of 95D cells were exacted using protein extraction reagent (Thermo Scientific ... WebJan 31, 2024 · The biotin-coupled RNA complex was pull-downed by incubating the cell lysates with streptavidin magnetic beads (Life Technologies, USA) for another 4 h. The … cycloplegics and mydriatics

Biotin-based Pulldown Assay to Validate mRNA Targets of

WebMay 15, 2024 · They found that miR-193a-3p functioned as a tumor suppressor in PCa cells, through bioinformatic prediction and biotin-coupled miRNA capture studies. Functional studies showed that miR-193a-3p reduced MCL1 expression, to suppress proliferation, migration, and invasion in cancer cells . WebNov 23, 2024 · Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124. In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in … WebJun 21, 2024 · In addition, luciferase reporter, biotin‑coupled miRNA capture and RNA immunoprecipitation assays revealed that circZYG11B could regulate RGC function through circZYG11B/microRNA‑620/PTEN signaling. Clinically, RT‑qPCR assays demonstrated that circZYG11B expression was markedly increased in the aqueous humor of patients with … cyclopithecus

circCHST15 is a novel prognostic biomarker that promotes clear cell

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WebMay 26, 2024 · Biotin-coupled miRNA capture. The 3′ end of biotinylated miR-556-5p and miR-1322 mimics or control RNA (Ribio, Guangzhou, China) were transfected into 1 × 10 6 HCT116 and DLD1 cells at a final concentration of 50 nM for 48 h before harvest. Then the cell pellet was incubated with 0.7 mL lysis buffer (5 mM MgCl 2. In vivo experiment WebJan 1, 2016 · Therefore, to identify all miR-96 targets (the miR-96 targetome) in an unbiased manner, we undertook a biotin-miRNA (bi-miR) capture and streptavidin pulldown … cyclopinclopanWebJan 10, 2024 · Biotin‐coupled miRNA capture The 50 μ m biotinylated miRNA mimic (GenePharma, Shanghai, China, sequence GCCCTTCATGCTGCCCAG) was … cycloplegic eye refraction

"WebJun 19, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as previously described 11. Briefly, 3′-end biotinylated miR-587 mimics (Ribio, Guangzhou, China) were ... " - Biotin-coupled mirna capture

Biotin-coupled mirna capture

mRNAs biotinylated within the 5′ cap and protected against …

WebMay 31, 2024 · Biotin-coupled miRNA capture The biotin-coupled miRNA pull-down assay was performed as described previously by Zheng and colleagues ( 16 ). Briefly, the 3′ end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPC-A1 cells at a final concentration of 20 nmol/L for 1 day. WebJan 16, 2024 · RNA immunoprecipitation (RIP) and biotin-coupled miRNA capture. g for 20 min. Western blot analysis. The total protein of 95D cells were exacted using protein extraction reagent (Thermo Scientific) with a cocktail of proteinase inhibitors (Roche Applied Science, Switzerland). Equal amount of total protein was separated by 10% sodium …

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WebDec 18, 2024 · Biotin-coupled miRNA capture The 3′ end biotinylated miRNA mimics or control RNA (Ribio, Guangzhou, China) were transfected into ccRCC cells for 48 h … WebInvitrogen streptavidin-coupled Dynabeads are a robust and versatile tool that can be used to capture specific RNA or DNA sequences and then pull them directly out of solution. …

WebDec 20, 2024 · Biotin-coupled miRNA capture. For biotin-coupled capture, approximately 5 × 10 6 BCa cells (T24 and 253 J-BV) were transfected with biotin-miR135a-5p or negative control (NC) by …

WebJul 21, 2024 · Biotin-coupled miRNA capture. Biotin-coupled miRNA pulldown assay was performed as described previously [27, 28]. In brief, 3′-end biotinylated miR-1-3p mimics (GenePharma) were transfected into YTS-1 cells using GP-transfect Mate reagent (GenePharma). Cells were lysed with lysis buffer ... WebJul 17, 2024 · Biotin-coupled miRNA capture. Because PTEN plays an important role in regulating cell proliferation and is one of the miR-181b-5p target genes, a pull-down assay was performed to investigate whether miR-181b-5p directly binds to PTEN. Briefly, HPDLSCs were transfected with biotinylated miRNA mimics or nonsense control (NC) …

WebFeb 12, 2024 · Biotin-coupled miRNA capture assay. The biotin-labeled circHIPK3 and control probes were synthesized by GenePharma (Shanghai, China). In brief, biotin-labeled circHIPK3 and control probes were …

WebAug 7, 2024 · The capture probe, 35 to 50 base-long and coupled to biotin on its 3′ end, binds the 5′ half of the miRNA. The detection probe is composed of a 3′ end complementary to the miRNA and a chain of fluorescently labeled RNA sequences, forming a fluorescent barcode associated with the target miRNA. cycloplegic mechanism of actionWebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... cyclophyllidean tapewormsWebJan 31, 2024 · Biotin-coupled probe pull down assay, Biotin-coupled miRNA capture, Fluorescence in situ hybridization and Luciferase reporter assay were conducted to confirm the relationship between the circ-ITCH and the microRNA. Results: In the present study, we found that circ-ITCH, is down-regulated in BCa tissues and cell lines. BCa patients with … cycloplegic refraction slideshareWebNov 17, 2024 · In addition, a biotin-coupled miRNA capture assay was performed using a biotin-coupled miR-185-3p probe, followed by qRT-PCR. It was observed a fourfold enrichment of circ_0008494 in the miR … cyclophyllum coprosmoidesWebIn the biotin-coupled miRNA capture assay, the direct interaction between HIPK3 and miR-194 was verified. While upregulating HIPK3 promoted NSCLC cell proliferation, co-transfection with both HIPK3 and miR-194 abrogated the tumor-promoting effects of HIPK3. Altogether, these data corroborated that HIPK3 and miR-149 exert opposing roles in … cyclopiteWebApr 8, 2024 · Subsequently, a biotin-coupled miRNA capture assay was performed to detect the sponge effect between hsa_circRNA_0088036 and miR-140-3p. BCa cells transfected with oehsa_circRNA_0088036 were also ... cyclop junctionsWebJul 13, 2024 · 2.13 Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was carried out as previously described. 17, 18 Briefly, the 3′-end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPCA1 cells at a final concentration of 20 nmol/L for 1 day. The biotin-coupled RNA complex was pulled … cycloplegic mydriatics