WebThe main sites of incision during repair of DNA containing a 1,3-intrastrand d (GpTpG)-cisplatin crosslink are at the 16th–20th phosphodiester bond 5′- and the 8th–9th phosphodiester bond on the 3′-side of the lesion (3). Based on techniques Mahmud K. Shivji et al., 2006, Springer Protocols Rheology of Mucin Web3.1 Isolation of Cisplatin DNA-Cross-linked Proteins 1. Rinse 1 × 10 7 cells in 30 mL of cold Hank’s buffer. 2. Centrifuge at 50g for 5 min at room temperature. 3. Repeat the rinse two more times. 4. Decant the Hank’s buffer from the cell pellet and add 10 mL of 1 m M cisplatin solution to the pellet. 5. Incubate at 37°C for 2 h with shaking. 6.
IJMS Free Full-Text Comet Assay Profiling of FLASH-Induced …
WebDec 15, 1995 · The widely used antitumor drug cis -diamminedichloroplatinum (II) (cisplatin or cis -DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, … WebFeb 18, 2012 · Cisplatin (cis-diamminedichloroplatinum(II)) causes crosslinking of DNA at AG and GG sites in cellular DNA, inhibiting replication, and making it a useful anti-cancer drug. Several techniques have been used previously to detect nucleic acid damage but most of these tools are labour-intensive, time-consuming, and/or expensive. Here, we describe … cryptocurrency financial times
Repair of cisplatin-induced DNA interstrand ... - Oxford Academic
WebFeb 28, 2007 · For instance, cisplatin has been reported to cross-link chromosomal proteins, including histones or cytokeratins, to DNA (8, 9). Interestingly, DPCLs have been shown to play an important role in cytotoxicity within the clinical dosage range of cisplatin ( 10 ), but frequency of these ternary complexes depends on the cell type ( 11 ) and time of ... WebJul 6, 2016 · An abnormal differentiation state is common in BRCA1-deficient mammary epithelial cells, but the underlying mechanism is unclear. Here, we report a convergence between DNA repair and normal, cultured human mammary epithelial (HME) cell differentiation. Surprisingly, depleting BRCA1 or FANCD2 (Fancon … Web• Introduction 1. Features of inorganic drugs 2. Approaches in Drug Discovery • Anticancer metal complexes– cisplatin • Some other inorganic drugs 1,2-Intrastrand Cross-Link 1,2-Interstrand Cross-Link 1,2-链内交联 1,2-链间交联 √ STRUCTURAL CHANGES IN DNA: 引起DNA解旋和弯曲 B-DNA 1,2-Intrastrand Crosslink 1,2-Interstrand ... durhamworks