WebDec 19, 2024 · Fragments were purified by ethanol precipitation then labeled with dCTP-Cy5 (Cytiva PA55021) by end-repair with Klenow fragment DNA polymerase (3′−5′ exo-; NEB). ... Cy5-DNA labeling ... Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting. Quick SDS-PAGE analysis: Prelabel protein samples with ready-to-use Cy5 dye for quick, sensitive detection without gel staining and destaining.
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WebRaw nanopore FAST5, fastq, and mapped bed files will be deposited to in the NCBI Trace Archive prior to publication of this manuscript and are available upon request. Summary: Pioneer transcription factors engage nucleosomal DNA in chromatin to initiate gene regulatory events that control cell fate1. WebApr 4, 2024 · Cy5.5 (Cyanine 5.5) 是一种发近红外(NIR)荧光的花青素荧光染料。它的消光系数高，荧光也很亮，并且对pH不敏感，并且由于它的荧光波长(Em：~700nm)恰好处于 … marriage certificate soft copy
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WebThe ready-to-use Cy5 NHS ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples. Samples are pre-labeled using Cy5 dye reagent prior to electropho- resis. This enables direct detection of pre-labeled proteins in the sample, eliminating the need for post-staining the gel. WebCy ® 5 Mono NHS Ester has been used in microarray experiments. [ 1] For labelling via amine groups. CyDyes are suitable for use in applications such as fluorescence … WebPerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. PerCP-Cy5.5 is optimized for use with a … nbc sports stream chicago