Phosphinothricin ppt
WebDL-Phosphinothricin is a herbicide that has been used as a selection agent for the genes bar or pat and is the active ingredient in Basta®, Glufosinate Ammonium, PPT, 2-amino-4- … WebAbout 50 =B5M phosphinothricin (PPT, the active ingredient in Basta) is sufficient for the selection *** I used pure phosphinotricin for selection of BASTA resistant Arabidopsis seedlings harboring a p35S-bar gene. This worked nicely at a concentration of 0.05 mM.
Phosphinothricin ppt
Did you know?
WebJan 5, 2024 · 1. Introduction. L-Phosphinothricin (L-PPT; L-2-amino-4-(hydroxy(methyl)-phosphonoyl)butanoic acid) is the active ingredient of many commercial herbicides used worldwide such as Basta, Buster, Challenge, Finale, Harvest, and Ignite [1].As L-PPT resembles L-glutamic acid (L-Glu), it functions as a false substrate and irreversibly blocks … WebAug 3, 2024 · To determine the structure of Hpy GS, crystals of the apo- and substrate-bound forms in complex with ATP and phosphinothricin (PPT) were obtained. The apo structure of Hpy GS (Hpy GS apo S ...
WebPhosphinothricin (PPT) is the active ingredient in bialaphos, which specifically inhibits glutamine synthetase in land plants. We isolated a novel PPT-resistant gene from a soil … WebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, …
WebPhosphinothricin acetyltransferase ( EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin … WebJan 1, 1995 · The bialaphos resistance gene (bar) is part of the biosynthesis pathway of bialaphos 6.The strains Streptomyces hygroscopicus and Streptomyces …
WebApr 1, 2024 · L-phosphinothricin (L-PPT) is a competitive and environmentally friendly herbicide. To develop an efficient approach for synthesis of l-PPT, a kinetic resolution route with a novel...
WebJan 7, 2024 · In BY-2 cells, phosphinothricin had been already used by Nakamura et al. ( 2010 ), who recommended 2 mg/l PPT for selection. However, this protocol did not work in our hands, and later on, PPT was even considered unsuitable for suspension cultures by some authors (Seifertová et al. 2014 ). how to say spider in frenchWebShowing 1-1 of 1 result for "phosphinothricin" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (2) … how to say spicy in spanishWebOct 28, 2024 · Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of … how to say spiderman in frenchWebAug 16, 2007 · The present investigation describes a PPT-based selection system for alfalfa transformation that uses the phosphinothricin acetyl-transferase ( pat) gene as a selectable marker and 5.0 mg l −1 of bialaphos as the selective agent. how to say spider-manWebSep 12, 2014 · 此可读框架通过组成型启动子CaMV35S启动，携带PPT乙酰基转移酶(PAT)基因作为选择标记基因，并将构建的表达载体导入根癌农杆菌EHA105。确定烟草转化的潮霉素临界筛选浓度为50mg，头孢噻肟钠的抑菌浓度为200mg。 how to say spicy in thaiWebWild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to the ammonium salt of l-phosphinothricin (PPT, also called glufosinate), an irreversible inhibitor of glutamine synthetase activity and the main active component of the herbicide BASTA (AgrEvo, Frankfurt am Main, Germany). Under the same conditions, however, this strain … how to say spider in italianWebApr 1, 2024 · Phosphinothricin (PPT), also known as glufosinate, is an unnatural amino acid with the distinctive structural characteristic of a phosphinic acid moiety. PPT is a non-selective and broad-spectrum herbicide [ 1]. northland resort walker mn